mouse anti-tpx2  (Cell Signaling Technology Inc)

Journal: Cell reports

Article Title: An Aurora kinase A-BOD1L1-PP2A B56 axis promotes chromosome segregation fidelity

doi: 10.1016/j.celrep.2025.115317

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Article Snippet: mouse anti-TPX2 , Cell Signaling Technology , Cat# D9Y1V.

Techniques: Virus, Recombinant, Staining, shRNA, Cloning, Plasmid Preparation, Software

Journal: bioRxiv

Article Title: Myosin 10 uses its MyTH4 and FERM domains differentially to support two aspects of spindle pole biology required for mitotic spindle bipolarity

doi: 10.1101/2023.06.15.545002

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Article Snippet: Primary antibody , mouse anti-Tpx2 , Santa Cruz Biotechnology , Cat#sc-53775 , Immunofluorescence (1:200).

Techniques: Recombinant, CRISPR, Imaging, Fluorescence, Labeling, Concentration Assay, Western Blot, Transfection, Cloning, Sequencing, Control, Immunofluorescence, Software

Journal: Cell & Bioscience

Article Title: Phosphorylation of adducin-1 by TPX2 promotes interpolar microtubule homeostasis and precise chromosome segregation in mouse oocytes

doi: 10.1186/s13578-022-00943-y

Figure Lengend Snippet: Expression and subcellular localization of TPX2 during mouse oocyte meiotic maturation. A The protein content of TPX2 in oocytes at the GV, GVBD, MI, ATI, and MII stages was detected by immunoblotting. Protein loading was verified by the detection of β-actin. The molecular mass of target proteins is indicated on the right. B TPX2 was quantified for four independent repeats (normalized to β-actin, arbitrary units). Different lowercase letters above bars indicate statistical difference at p < 0.05 by ANOVA with the Tukey test. C Immunofluorescent localization of TPX2 in meiotic oocytes at various stages. D Spatial distribution of TPX2 and spindle microtubules in oocytes at different meiotic maturation stages. E Oocytes at the MI and MII stages were treated with taxol and then double-stained for TPX2 and α-tubulin. F Oocytes at the MI and MII stages were exposed to nocodazole and then co-stained for p-ADD1 and α-tubulin. Red, ADD1; green, α-tubulin; blue, DNA; Merge, overlapping of red, green, and blue. Bar, 20 μm

Article Snippet: The rabbit polyclonal anti-ADD1 antibody (Catalog# NBP1-48,611) and the mouse monoclonal anti-TPX2 antibody (Catalog# NBP2-67,265) were purchased from Novus Biologicals.

Techniques: Expressing, Western Blot, Staining

Journal: Cell & Bioscience

Article Title: Phosphorylation of adducin-1 by TPX2 promotes interpolar microtubule homeostasis and precise chromosome segregation in mouse oocytes

doi: 10.1186/s13578-022-00943-y

Figure Lengend Snippet: Effects of TPX2 depletion on oocyte maturation, spindle assembly, chromosome alignment, and the expression of p-ADD1 and ADD1. A Immunoblot probed with anti-TPX2 antibody demonstrating the depletion efficiency of TPX2-specific morpholino (MO-TPX2) in mouse oocytes. B TPX2 protein content in control-MO and TPX2-MO-injected oocytes was quantified for three independent repeats. C Representative images of oocytes cultured in vitro for 16 h after treatment with control-MO or TPX2-MO. Bar, 100 μm. D Depletion of TPX2 caused meiotic cell cycle arrest in MI in mouse oocytes. E Subcellular localization of p-ADD1, spindle morphologies, and chromosome alignment after 16 h of maturation culture in oocytes injected with control-MO or TPX2-MO. Bar, 20 μm. F The rate of the oocyte with a normal spindle, aberrant spindle, or no spindle was quantified in the control-MO and ADD1-MO-injected oocytes. G The rate of the oocyte with normal p-ADD1 localization, abnormal p-ADD1 localization, or no p-ADD1 localization was recorded in the control and ADD1 disrupted oocytes. H The depletion of TPX2 downregulated the phosphorylation of ADD1 at S726 in mouse oocytes. I The protein level of p-ADD1 in control and TPX2-depleted oocytes was quantified for three independent repeats. J Effect of TPX2 depletion on ADD1 protein expression in mouse oocytes. (K) ADD1 protein levels in control-MO and TPX2-MO-injected oocytes were quantified for three independent replicates. In B , I , K , each bar denotes the mean ± SEM of the three independent repeats, and different uppercase letters or lowercase letters above columns indicate statistical difference at p < 0.01 or p < 0.05 by student’s t -test, respectively. Whereas bars that do not share the same uppercase letter are significantly different at p < 0.01 by the chi-square test in D , F , G

Article Snippet: The rabbit polyclonal anti-ADD1 antibody (Catalog# NBP1-48,611) and the mouse monoclonal anti-TPX2 antibody (Catalog# NBP2-67,265) were purchased from Novus Biologicals.

Techniques: Expressing, Western Blot, Control, Injection, Cell Culture, In Vitro, Phospho-proteomics

Journal: Cell & Bioscience

Article Title: Phosphorylation of adducin-1 by TPX2 promotes interpolar microtubule homeostasis and precise chromosome segregation in mouse oocytes

doi: 10.1186/s13578-022-00943-y

Figure Lengend Snippet: Schematic illustration of the molecular mechanism by which ADD1 regulates spindle assembly in mouse oocytes. ADD1 is phosphorylated by TPX2 and aggregates to the spindle poles to regulate the homeostasis of interpolar microtubules to ensure the formation of functional spindles and the proper segregation of chromosomes in mouse oocytes

Article Snippet: The rabbit polyclonal anti-ADD1 antibody (Catalog# NBP1-48,611) and the mouse monoclonal anti-TPX2 antibody (Catalog# NBP2-67,265) were purchased from Novus Biologicals.

Techniques: Functional Assay